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NociOcular Assay Image
to assess eye stinging   



Ophthalmic Products

The NociOcular Assay predicts the potential of materials such as cosmetics, sunscreens, surfactants, and ophthalmic products to cause eye sting. The assay relies on the expression of functional Transient Receptor Potential Vanilloid type 1 (TRPV1) channels in a clone of the human SH-SY5Y neuroblastoma cell line. The TRPV1 channel is a well characterized pain receptor that is present in tissues associated with the eye. This channel can be activated by chemical stimuli and is thought to be a general mediator of chemically induced pain on the surface of the eye, which can cause a stinging sensation in a clinical setting.

NociOcular: SH-SY5Y neuroblastoma cells used for the NociOcular Assay
NociOcular: Serial dilution of a test article loaded on a 96-well plate to be dosed onto the cells by the FlexStation machine
NociOcular: The FlexStation Fluorometer doses the cell plate with the compound and reads the fluorescence intensity.

Although several in vitro eye irritation models exist, no current in vitro assay has demonstrated the ability to predict if products which may come in contact with the eyes will cause a stinging sensation. Data support that the TRPV1 channel is a principle mediator of eye sting and that the NociOcular assay may serve as a simple bioassay to determine sensory response in the eye. The NociOcular assay has been shown to be capable of detecting eye sting potential in a number of product classes (see the Publications table below) and can be a useful pre-clinical screening tool.

The current prediction model for the assay is based on studies with surfactant ingredients and formulations. Current work is focusing on the expansion of the applicability of the assay through investigation of non-surfactant product types such as creams and lotions (eg. sunscreens). Please view the recording of our webinar on the assay and contact us to discuss how the NociOcular assay for eye sting can be incorporated into your testing strategy.

Webinar Slides

Nociocular Webinar Slides Cover
Our webinar slide presentation of the NociOcular Assay is available for download here!

Publications & Articles

Type Document Published
Poster Using the Novel NociOcular Assay to Predict the Eye Sting Potential of Shampoos and Sunscreen Products 2015-03-23
Poster Evaluation of TRPV1 Activity to Assess the Eye Stinging Potential of Cosmetic Formulations 2015-03-23
Newsletter Predicting Eye Stinging using the Novel NociOcular Assay: Investigating Surfactant, Sunscreen, and Ophthalmic Products 2014-10
Newsletter Article NociOcular Assay: A Novel In Vitro Assay to Assess Eye Stinging Potential 2013
Journal Article Using Novel In Vitro NociOcular Assay Based on TRPV1 Channel Activation for Prediction of Eye Sting Potential of Baby Shampoos 2012-10-01